Part:BBa_K3453124

Rosewood DmMatK Trigger 2.1 transcription unit

This part is a transcription unit of the Rosewood DmMatK Trigger 2.1 (BBa_K3453024) for sequence-based detection of rosewood. The transcription is controlled by the T7 promoter (BBa_K2150031) and the strong SBa_000587 synthetic terminator (BBa_K3453000).

Usage and Biology

A toehold switch is an RNA–based device containing a ribosome binding site (RBS) and an ATG start codon embedded in a hairpin structure that blocks translation initiation [1]. The hairpin can be unfolded upon binding of a trigger RNA therefore exposing the RBS and the ATG start codon permitting translation of the reporter protein (Figure 1).

Figure 1. Toehold switches principle.

This part is a transcription unit of BBa_K3453024 which is a trigger for the Rosewood DmMatK Toehold Switch 2.1 (BBa_K3453014).

This part proved to be functional: the transcribed rosewood RNA was able to bind to the corresponding toehold switches and a readable output was generated.

Full results are available on the BBa_K3453114 page in the registry.

References

[1] Green AA, Silver PA, Collins JJ, Yin P. Toehold switches: de-novo-designed regulators of gene expression. Cell (2014) 159, 925-939.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 46
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 46
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 46
  Illegal BamHI site found at 57
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 46
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 46
  
• 1000
  COMPATIBLE WITH RFC[1000]